Comparison of T-matrix method with discrete sources method applied for total internal reflection microscopy
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چکیده
منابع مشابه
Total internal reflection microscopy: examination of competitive schemes via discrete sources method
The discrete sources method has been applied to perform a computer simulation analysis of different total internal reflection microscopy schemes. It has been found that the positioning of the objective lens beneath a glass prism can provide a considerable advantage for determination of the particle–film distance.
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The goal in fluorescence microscopy is to detect the signal of fluorescently labeled molecules with great sensitivity and minimal background noise. In epifluorescence microscopy, it is difficult to observe weak signals along the optical axis, owing to the overpowering signal from the out-of-focus particles. Confocal microscopy uses a small pinhole to produce thin optical sections ( 500 nm), but...
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ژورنال
عنوان ژورنال: Journal of Quantitative Spectroscopy and Radiative Transfer
سال: 2007
ISSN: 0022-4073
DOI: 10.1016/j.jqsrt.2007.01.042